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Enzyme electrophoresis
can be performed to determine the genetic variation of organisms. A
population of organisms of the same species, may have only one form of a given
enzyme. If, however, there are two enzymes that differ in charge they can be
distinguished by electorophoresis. These enzyme variants are a
result in small changes in the DNA sequence of protein coding genes, altering
what amino acids are incorporated into the proteins. When a slurry of a tissue
is placed in a starch gel and subjected to an electrical current, the enzyme
molecules with different electrical charges with migrate through the gel at
different rates. This procedure is known as electrophoresis. After
electrophoresis the gel is bathed in cofactors for a particular enzyme and dye
that precipitates when the cofactor and enzyme react. This allows the
observation of the mobility of each enzyme based on the position in the gel.
This data is then used to determine allele frequency in populations of species.
These two forms of the same enzyme are called isozymes. The two isozymes may be different because they are two different enzymes, each coded for by its own genetic locus. This occurs if the genome had two copies of genes for functionally identical enzymes. Alternatively, the isozymes could correspond to charge variations of the gene products from a single genetic locus, i.e., two alleles of the same gene, in which case the charge isomers are called allozymes.
Allozyme
data are used to track and to characterize genetic variation in natural
populations. An individual can be either homozygous or heterozygous at a given
allozyme locus. If all of the individuals in a population are homozygous for
the same charge isomer, then the population is said to be monotypic,
lacking in genetic variation for that enzyme. If some of the individuals in a
population are homozygous for one allozyme and others are homozygous for the
other allozyme, this indicates genetic variation within the population for that
enzyme. Populations that are highly inbred or have recently been through a
population bottleneck usually display a low degree of allozyme variation.
If there is allozyme variation in the population, then one can calculate (using the Hardy-Weinberg law) the expected frequencies of heterozygotes. These values can be used in aiding conversation efforts. Knowing the allele frequency allows researchers to to determine genetic differentiation among populations.